Subunits of the GPI transamidase complex localize to the endoplasmic reticulum and nuclear envelope in <i>Drosophila</i>

A total of 10–20% plasma membrane proteins are anchored by glycosylphosphatidylinositol (GPI). GPI is attached to transamidase (GPI‐T), which contains five subunits named PIGK, PIGS, PIGT, PIGU, and GPAA1. We previously reported that PIGT localizes near the nucleus in Drosophila . However, localizations other four remain unknown. Here, we show a catalytic subunit GPI‐T, mainly endoplasmic reticulum (ER), while localize nuclear envelope (NE) ER. The NE/ER localization ratio PIGS differs between cell types developmental stages. Our results suggest GPI‐T catalyzes attachment ER may have unknown functions NE.